Circular Dichroism Spectrophotometer (CD) J-1500, Jasco

Circular Dichroism Spectrophotometer (CD) J-1500, Jasco, CEITEC Biomaterials

Description

  • Based on the principle of circular dichroism (CD) - difference in the absorption of left-handed circularly polarised light (L-CPL) and right-handed circularly polarised light (R-CPL)
  • Applicable for molecules containing one or more chiral chromophores (light-absorbing groups)
  • Measurements carried out in the visible and ultra-violet region of the electro-magnetic spectrum monitor electronic transitions, a circular dichroism signal can be positive or negative, depending on whether L-CPL is absorbed to a greater extent than R-CPL (CD signal positive) or to a lesser extent (CD signal negative)
  • Fast, quantitative and non-destructive photometric technique that is used extensively to study chiral molecules of all types and sizes
  • Analysis of bulky biological molecules with regard to their stability, structure and interactions of nucleic acids and proteins.


Use

  • Determination of protein secondary structure
  • Determination of optical purity
  • Analysis of the tertiary structure of proteins and conformational changes
  • Comparison of the secondary and tertiary structures of wild-type and mutant proteins
  • Binding studies involving conformational changes
  • Nucleic acid structure and changes upon binding or melting
  • Thermodynamic parameters from temperature dependencies of CD signals
  • Kinetics of conformational changes on msec timescales
  • Quantitative analysis of pharmaceuticals


Specification

  • Variety of samples, from liquids to films to solid-states
  • High optical throughput
  • Double prism monochromator providing low stray light
  • Wide dynamic range standard PMT detector (163 – 950 nm) and an optional InGaAs detector (up to 1600 nm)
  • Highly efficient nitrogen purge system
  • Digital signal processing
  • Simultaneous Multi-Probe: CD, LD, HT, DC, absorbance, fluorescence
  • Multi-position cells to run thermal melts
  • Measurements on up to 192 samples without user intervention
  • Dedicated stopped-flow systems that can measure both CD and fluorescence


Contact

​Assoc. Prof. Lucy Vojtová, Ph.D.
Research Group Leader
lucy.vojtova@ceitec.vutbr.cz
biomaterials.ceitec.cz